BT501 FINAL TERM SOLVED PAPERS

BT501 FINAL TERM SOLVED PAPERS
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A thermal cycler is a device that performs polymerase chain reaction amplification reactions. Typically, the three main events are repeated for 30-40 cycles to achieve a detectable amount of product at the end of the cycles. The automated system performs cyclical temperature changes required for enzymatic amplification of specific DNA segments v in vitro using this PCR.

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BT501 FINAL TERM SOLVED PAPERS
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After entering the host, phenotypic changes should occur – another important feature is that the vector DNA should provide additional phenotypic changes in the host cells, making it easier to recognize transformed cells.
Multiple cloning site with unique restriction site – A short stretch of DNA on a vector DNA containing a restriction site for a possible insertion site for foreign DNA is desirable.

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Vehicle DNA is required to transfer foreign DNA to create a recombinant construct that should allow easy amplification of the chimeric DNA in the host. Vehicle DNA used for this purpose in genetic engineering is known as a “vector”. Criteria for a good vector: Vector DNA has two main responsibilities in a particular host strain.

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The device has a heat block with holes where the tubes containing reaction mixtures can be inserted. The cycler changes the temperature of the block in discrete, pre-programmed steps using the Peltier effect.

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