BT102 FINAL TERM SOLVED PAPERS

BT102 FINAL TERM SOLVED PAPERS
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Microbiology:

Physically attach the sample to the slide. Maintain internal and external structures in place. Physical method: Heat Chemical methods: Ethanol or formaldehyde Smudge staining. The stain is applied and then washed off with water.  The slide is air-dried or blotted.

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BT102 FINAL TERM SOLVED PAPERS
BT102 FINAL TERM SOLVED PAPERS
BT102 FINAL TERM PAST PAPERS:

DNA is not enclosed in a nuclear membrane.  Chromosome: usually one, circular.DNA is not associated with histones.  Non-membrane closed organelles.  The cell wall contains peptidoglycan, a complex carbohydrate.
Divide by binary fission.

BT102 FINAL TERM PAST PAPERS BY MOAAZ:

Various bacterial staining options are available.  Positive/negative staining  Differential staining. Special coloring  Malachite green: Primary stain  Steam for 5 minutes.  Washed with water.  Counterstained with safranin.

BT102 FINAL TERM SOLVED PAPERS:

The slide is washed with alcohol. – Bleaching agent  Gram-positive bacteria retain crystal violet. Gram-negative bacteria appear colorless. Crystal violet and iodine form a complex in the cytoplasm. The peptidoglycan layer is thicker in gram-positive bacteria and CV-I is preserved.  The alcohol is rinsed off and stained with safranin.

BT102 FINAL TERM PAST PAPERS MEGA FILES